Research services
Protein Expression & Purification
In silico drug design
X-ray crystal structure
Protein Expression & Purification
개발공정 및 서비스범위
Protein Construct Design
- Duration: ~ 2 weeks
- pET vector system
(pET21a, pET24a, pET28a, pET32a, etc.) - Amino acid sequence is provided
Expression test
- Duration: ~1 month
- Expression system
- E. coli
- Mammalian cell (CHO)
- SDS-PAGE result is provided
Cell culture
- Duration: ~1 month
- Scale: ~20 L
Protein purification
- Duration: ~ 2 months
- Chromatography
- – Affinity
- – IEX
- – SEC
- Purity > 90%
- SDS-PAGE and Analytical SEC profile are provided
In silico drug design
Protein drug design
- Mediate protein-protein interaction
- Enhancing protein stability
- Peptide mimic compound design
Small-molecule drug design
- Protein-ligand interaction prediction
- Ligand binding site prediction
- Lead optimization
X-ray crystal structure
- To obtain a three-dimensional structure of proteins
- To analysis function of proteins or protein-ligand interactions
- To use modifying drugs for enhancing potency or specificity
Crystallization
- Initial crystal condition screening
- Optimize conditions for crystal growth
- Co-crystallization or soaking of ligands
X-ray crystallography
- X-ray diffraction
- Scaled & merged diffraction data
- Phasing or molecular replacement
- Model refinement