Research services
Protein Expression & Purification
In silico drug design
X-ray crystal structure
Protein Expression & Purification
개발공정 및 서비스범위
발현용 벡터 cloning 서비스
- 소요기간: ~2주
- 기본 제공 벡터
- pET21a
- pET24a
- pET32a
- Agarose gel 및 sequencing 결과 제공
발현 평가
- 소요기간: ~1주
- Expression system
- E. coli
- Mammalian cell (CHO)
- SDS-PAGE 결과 제공
배양
- 소요기간: ~1주
- Scale: 0.5-20 L
정제
- 소요기간: ~1주
- Affinity
- IEX
- HIC
- SEC
- 순도 >90-98%
- SDS-PAGE, Analytical SEC
결과 제공
In silico drug design
Protein drug design
- Mediate protein-protein interaction
- Enhancing protein stability
- Peptide mimic compound design
Small-molecule drug design
- Protein-ligand interaction prediction
- Ligand binding site prediction
- Lead optimization
X-ray crystal structure
- To obtain a three-dimensional structure of proteins
- To analysis function of proteins or protein-ligand interactions
- To use modifying drugs for enhancing potency or specificity
Crystallization
- Initial crystal condition screening
- Optimize conditions for crystal growth
- Co-crystallization or soaking of ligands
X-ray crystallography
- X-ray diffraction
- Scaled & merged diffraction data
- Phasing or molecular replacement
- Model refinement